Protective and Therapeutic Role of Ginkgo Biloba Extract Through TRPV1 Channels in an in Vitro Alzheimer’s Disease Model

Ahmet ÖZŞİMŞEK , İshak Suat ÖVEY , Ertan KARAÇAY , Burak YULUĞ
2023 September - 60 (3)


• Ginkgo biloba has TRPV1 channels-via antiapoptotic
effect in vitro model of AD.
• Ginkgo biloba reduces intracellular Ca2+ and ROS levels
mediated by TRPV1 channels.
• Ginkgo biloba has a disease-protective effect when
administered before AD is formed.


Introduction: The effect of Ginkgo biloba (GB) on mitochondriadependent
TRPV1 ion channels in neuroblastoma cells was investigated
by creating an Alzheimer’s disease (AD) model.
Methods: Okadaic acid was applied on SH-SY5Y cells to create an AD
model. After cellular differentiation, the study was organized with the
seven main groups, examining the effect of GB on calcium depended
TRPV1 channels in neuroblastoma cells AD, has been established in vitro.
Results: The higher Ca2+ concentration was detected in the GB+AD, AD
and AD+GB groups when compared with the control (p<0.001). The
Ca2+ level was lower in GB+AD and AD+GB groups than in the AD group
(p<0.001). Also, cytosolic Ca2+ concentration was lower in the GB+AD
than in the AD+GB group (p<0.05), the apoptosis and intracellular reactive
oxygen species (ROS) values were higher in the GB+AD, AD and AD+GB
groups than in the control (p<0.001). The apoptosis and intracellular
ROS values were higher in AD group than in the GB+AD and AD+GB
group (p<0.001) and the apoptosis level was higher in AD+GB group
than GB+AD group (p<0.001) and the mitochondrial depolarization,
caspase 3 and caspase 9 levels were higher in the GB+AD, AD and AD+GB
groups when compared to the control group (p<0.001). Also, the values
were lower in the GB+AD group, AD group and AD+GB groups when
compared with the GB+AD+capsazepine group, AD+capsazepine group
and AD+GB+capsazepine respectively (p<0.001).
Conclusion: These results show us that GB has a protective effect besides
its therapeutic effect in Alzheimer’s disease via TRPV1 channel.
Keywords: Alzheimer disease, Ginkgo biloba, neuroblastoma cells, ROS
(reactive oxygen species), TRPV1.